ALDH1A1 Polyclonal Antibody
Catalogue Numbers: BS60846-50, BS60846-100
Sizes: 50µl, 100µl
Alternative Name: Retinal dehydrogenase 1; RALDH 1; RalDH1; ALDH-E1; ALHDII; Aldehyde dehydrogenase family 1 member A1; Aldehyde dehydrogenase, cytosolic; ALDH1A1; ALDC; ALDH1; PUMB1
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P00352
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB
All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200
Background: Aldehyde dehydrogenases (ALDHs) mediate NADP+-dependent oxidation of aldehydes into acids during the detoxification of alcohol-derived acetaldehyde; metabolism of corticosteroids, biogenic amines and neurotransmitters; and lipid peroxidation. ALDH1A1, also designated retinal dehydrogenase 1 (RalDH1 or RALDH1), aldehyde dehydrogenase family 1 member A1, aldehyde dehydrogenase cytosolic, ALDHII, ALDH-E1 or ALDH E1, is a retinal dehydrogenase that participates in the biosynthesis of retinoic acid (RA). There are two major liver isoforms of ALDH1 that can localize to cytosolic or mitochondrial space. The ALDH1A2 (RALDH2, RALDH2-T) gene produces three different transcripts and also catalyzes the synthesis of RA from retinaldehyde. ALDH1A3 (ALDH6, RALDH3, ALDH1A6) is a 37 kb gene that consists of 13 exons and produces a major transcript of approximately 3.5 kb most abundant in salivary gland, stomach and kidney. ALDH3A1 (stomach type, ALDH3, ALDHIII) forms a cytoplasmic homodimer that preferentially oxidizes aromatic aldehyde substrates. ALDH genes upregulate as a part of the oxidative stress response, and appear to be abundant in certain tumors that have an accelerated metabolism toward chemotherapy agents.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -22°C long term. Avoid freeze-thaw cycles.
Specificity: ALDH1A1 polyclonal antibody detects endogenous levels of ALDH1A1 protein.
Bioworld Molecular Weight: ~ 54 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of ALDH1A1 polyclonal antibody at 1:500 dilution
Lane 1: HEK293T whole cell lysate
Lane 2: Raw264.7 whole cell lysate
Lane 3: NIH-3T3 whole cell lysate
Lane 4: PC12 whole cell lysate
Immunogen: A synthetic peptide corresponding to residues in Human ALDH1A1.
Conjugate: Unconjugated
Modification: Unmodified