Aldolase B (W148) Polyclonal Antibody
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Catalogue Numbers: BS3127-50, BS3127-100
Sizes: 50µl, 100µl
Swiss-Prot: P05062
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IHC
Background: Aldolase A is one of the most highly conserved enzymes known, with only about 2% of the residues changing per 100 million years. Aldolase B is regulated by the hormones insulin and glucagons and has been implicated in hereditary fructose intolerance disease. Aldolase C is 36 kDa polypeptide that is exclusively expressed in Purkinje cells. Aldolase C-positive Purkinje cells are organized in the cerebellum as stripes or bands that run from anterior to posterior across the cerebellum and alternate with bands of Aldolase C-negative Purkinje cells.
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Specificity: Aldolase B (W148) polyclonal antibody detects endogenous levels of Aldolase B protein.
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of Aldolase B (W148) pAb at 1:2000 dilution
Lane 1: AML-12 whole cell lysate (40ug)
Lane 2: The Kidney tissue lysate of Rat (10ug)
Lane 3: HepG2 whole cell lysate (40ug)
Lane 4: HEK293T whole cell lysate (20ug)
Lane 5: SGC7901 whole cell lysate (20ug), Immunohistochemistry (IHC) analyzes of Aldolase B (W148) pAb in paraffin-embedded human lung carcinoma tissue at 1:50,showing cytoplasmic and nuclear staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
Bioworld Molecular Weight: ~ 40 to 45 kDa
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Alternative Name: ALDOB; ALDOB; ALDB; Liver-type aldolase; Fructose-bisphosphate aldolase B
Immunogen: Synthetic peptide, corresponding to amino acids 121-170 of Human Aldolase B.
Conjugate: Unconjugated
Modification: Unmodified