ALOX15 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS80489-50, BS80489-100
Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P16050
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: IHC, IF/ICC
All Applications: IHC,1:50 - 1:200 | IF/ICC,1:50 - 1:200
Background: Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. It inserts peroxyl groups at C12 or C15 of arachidonate ((5Z,8Z,11Z,14Z-eicosatetraenoate producing both 12-hydroperoxyeicosatetraenoate/12-HPETE and 15-hydroperoxyeicosatetraenoate/15-HPETE. It may then act on 12-HPETE to produce hepoxilins, which may show proinflammatory properties (By similarity. Can also peroxidize linoleate ((9Z,12Z-octadecadienoate to 13-hydroperoxyoctadecadienoate/13-HPODE. May participate in the sequential oxidations of DHA ((4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoate to generate specialized pro-resolving mediators (SPMslike resolvin D5 ((7S,17S-diHPDHA and (7S,14S-diHPDHA, that actively downregulate the immune response and have anti-aggregation properties with platelets. Can convert epoxy fatty acids to hydroperoxy-epoxides derivatives followed by an intramolecular nucleophilic substitution leading to the formation of monocyclic endoperoxides. Plays an important role during the maintenance of self-tolerance by peroxidizing membrane-bound phosphatidylethanolamine which can then signal the sorting process for clearance of apoptotic cells during inflammation and prevent an autoimmune response.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Unmodification
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Immunohistochemistry of paraffin-embedded mouse intestin using ALOX15 Rabbit pAb at dilution of 1:25. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded mouse spleen using ALOX15 Rabbit pAb at dilution of 1:25. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded rat spleen using ALOX15 Rabbit pAb at dilution of 1:25. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol., Immunofluorescence analysis of MCF7 cells using ALOX15 Rabbit pAb at dilution of 1:25. Blue: DAPI for nuclear staining.
Alternative Name: ALOX15; 12-LOX; 15-LOX-1; 15LOX-1
Immunogen: Recombinant fusion protein of human ALOX15(NP_001131.3).
Conjugate: Unconjugated
Modification: Unmodified