Alpha-enolase Monoclonal Antibody
Catalogue Numbers: MB66612-50, MB66612-100
Sizes: 50µl, 100µl
Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Swiss-Prot: P06733
Host: Mouse
Reactivity: Human, Mouse, Rat, Monkey
Applications: WB
All Applications: WB (1/500 - 1/1000)
Background: Enolase is an important glycolytic enzyme involved in the interconversion of 2-phosphoglycerate to phosphoenolpyruvate. Mammalian enolase exists as three subunits: enolase-1 (α-enolase), enolase-2 (γ-enolase) and enolase-3 (β-enolase) that can form both homo- and heterodimers. Expression of the enolase isoforms differs in a tissue specific manner. Enolase-1 plays a key role in anaerobic metabolism under hypoxic conditions and may act as a cell surface plasminogen receptor during tissue invasion. Abnormal expression of enolase-1 is associated with tumor progression in some cases of breast and lung cancer. Alternatively, an enolase-1 splice variant (MBP-1) binds the c-myc promoter p2 and may function as a tumor suppressor. For this reason enolase-1 is considered as a potential therapeutic target in the treatment of some forms of cancer.
Purification and Purity: The antibody was purified by immunogen affinity chromatography.
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Recognizes endogenous levels of Alpha-enolase protein.
Extra Notes: Western blot analysis of Alpha-enolase expression in T47D (A), NIH3T3 (B), COS7 (C), C6 (D), Hela (E) whole cell lysates.
Bioworld Molecular Weight: ~ 47 kDa
Note: For research use only, not for use in diagnostic procedure.
Alternative Name: ENO1L1; MBPB1; MPB1; Alpha-enolase; 2-phospho-D-glycerate hydro-lyase; C-myc promoter-binding protein; Enolase 1; MBP-1; MPB-1; Non-neural enolase; NNE; Phosphopyruvate hydratase; Plasminogen-binding protein
Immunogen: Purified recombinant human Enolase-1 protein fragments expressed in E.coli.
Conjugate: Unconjugated
Modification: Unmodified