Alpha-fetoglobulin Monoclonal Antibody
Product: Mouse IgG2b kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Catalogue Numbers: MB66782-50, MB66782-100
Sizes: 50µl, 100µl
Swiss-Prot: P02771
Host: Mouse
Reactivity: Human
Applications: WB, IHC
Background: a-fetoprotein (AFP) is expressed in fetal liver at varying levels throughout development and is present only in trace amounts in normal adult tissues. AFP can be detected at abnormally high concentrations in hepatocellular carcinomas as well as in the plasma and ascitic fluid of adults with hepatoma. High AFP concentrations have been correlated with tumor cell growth, indicating
that AFP can serve as a tumor marker. AFP binds copper, nickel and fatty acids, and in some cases may bind serum albumin or estrogen. It has been demonstrated that the AFP promoter is a target for NF-1 (nuclear factor-1), HNF-1 (hepatocyte nuclear factor-1) and C/EBP transcription factors. While HNF-1 binding to the AFP promoter results in AFP expression, NF-1 binding results in a decrease in AFP promoter activity.
Product: Mouse IgG2b kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Specificity: Recognizes endogenous levels of Alpha-fetoglobulin protein.
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Alpha-fetoglobulin expression in human fetal liver (A) whole cell lysates., Immunohistochemical analysis of Alpha-fetoglobulin staining in human liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Bioworld Molecular Weight: ~ 68 kDa
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Alternative Name: HPAFP; Alpha-fetoprotein; Alpha-1-fetoprotein; Alpha-fetoglobulin
Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Alpha-fetoglobulin. The exact sequence is proprietary.
Conjugate: Unconjugated
Modification: Unmodified