Alpha2-adaptin/AP2A2 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS75277-50, BS75277-100
Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: O94973
Host: Rabbit
Reactivity: Human, Mouse
Applications: WB
All Applications: WB,1:500 - 1:2000
Background: The protein encoded by this gene is a subunit of the AP-2 adaptor protein complex, which is involved in linking lipid and protein membrane components with the clathrin lattice. This interaction supports the formation of clathrin-coated vesicles, and the encoded subunit aids in the process by binding polyphosphoinositide-containing lipids in the cell membrane.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Unmodification
Extra Notes: Western blot analysis of extracts of various cell lines, using Alpha2-adaptin/AP2A2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Note: For research use only, not for use in diagnostic procedure.
Bioworld Molecular Weight: 104kDa
Alternative Name: AP2A2; ADTAB; CLAPA2; HIP-9; HIP9; HYPJ
Immunogen: Recombinant fusion protein of human Alpha2-adaptin/AP2A2(NP_036437.1).
Conjugate: Unconjugated
Modification: Unmodified