AMACR Monoclonal Antibody
Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Catalogue Numbers: MB65874-50, MB65874-100
Sizes: 50µl, 100µl
Swiss-Prot: Q9UHK6
Host: Mouse
Reactivity: Human, Mouse, Rat
Applications: WB, IHC
Background: Catalyzes the interconversion of (R)- and (S)-stereoisomers of alpha-methyl-branched-chain fatty acyl-CoA esters (PubMed:7649182, PubMed:10655068, PubMed:11060359).
Acts only on coenzyme A thioesters, not on free fatty acids, and accepts as substrates a wide range of alpha-methylacyl-CoAs, including pristanoyl-CoA, trihydroxycoprostanoyl-CoA (an intermediate in bile acid synthesis), and arylpropionic acids like the anti-inflammatory drug ibuprofen (2-(4-isobutylphenyl)propionic acid) but neither 3-methyl-branched nor linear-chain acyl-CoAs (PubMed:7649182, PubMed:10655068, PubMed:11060359).
Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purification and Purity: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Specificity: Recognizes endogenous levels of AMACR protein.
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of AMACR expression in HepG2 (A), mouse kidney (B) whole cell lysates., Immunohistochemical analysis of AMACR staining in mouse prostate adenocancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Bioworld Molecular Weight: ~ 42 kDa
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Alternative Name: Alpha-methylacyl-CoA racemase; 2-methylacyl-CoA racemase
Immunogen: KLH-conjugated synthetic peptide encompassing a sequence of human AMACR. The exact sequence is proprietary.
Conjugate: Unconjugated
Modification: Unmodified