AMACR Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90050-50, BS90050-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q9UHK6(Human) O09174(Mouse)
Host: Rabbit
Reactivity: Human, Mouse
Applications: WB, ICC IHC FC
All Applications: WB:1:500
ICC:1:50-1:100
IHC:1:50-1:200
FC:1:50-1:100
Background: P504S, also known as AMACR (α-methylacyl-CoA racemase), 2-methylacyl-CoA racemase or RACE, is an enzyme belonging to the caiB/baiF CoA-transferase family. Localizing to the peroxisome and mitochondrion, P504S plays a role in the metabolism of branched-chain fatty acids and bile acid intermediates. More specifically, P504S catalyzes the conversion of pristanoyl-CoA and C27-bile acyl-CoAs to their (S)-stereoisomers which can then be degraded by peroxisomal β-oxidation. Mutations in the gene encoding P504S can lead to AMACR deficiency, a disease characterized by increased concentrations of pristanic acid that is associated with adult onset sensory motor neuropathy, and/or CBAS4 (congenital bile acid synthesis defect type 4), a disorder characterized by intrahepatic cholestasis, absence of cholic acid from bile, neonatal jaundice and bile duct deficiency. In addition, P504S is overexpressed in prostate cancer and is believed to be functionally important for prostate cancer cell growth.
Purification and Purity: Protein affinity purified.
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: AMACR polyclonal antibody detects endogenous levels of AMACR protein.
Bioworld Molecular Weight: 42 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of AMACR on different lysates using anti-AMACR antibody at 1/1,000 dilution.
Positive control:
Lane 1: Mouse liver tissue
Lane 2: SiHa
Lane 3: SW480
Lane 4: Human kidney tissue, ICC staining AMACR in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: AMACR, 2 arylpropionyl CoA epimerase, 2 methylacyl CoA racemase, 2-methylacyl-CoA racemase, Alpha methylacyl CoA racemase, Alpha methylacyl Coenzyme A racemase, Alpha methylacyl-CoA racemase deficiency, included, Alpha-methylacyl-CoA racemase, Amacr, AMACR deficiency, included, AMACR, CBAS4, Da1-8, EC 5.1.99.4, Macr1, Methylacyl CoA racemase alpha, RACE, RM,
Immunogen: Recombinant protein.
Conjugate: Unconjugated
Modification: Unmodified