Ambra1 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS6138-50, BS6138-100
Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q9C0C7
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IHC, IF/ICC
All Applications: WB,1:500 - 1:2000 | IHC,1:50 - 1:200 | IF/ICC,1:50 - 1:200
Background: Substrate-recognition component of a DCX (DDB1-CUL4-X-box E3 ubiquitin-protein ligase complex involved in cell cycle control and autophagy. The DCX(AMBRA1 complex specifically mediates the polyubiquitination of target proteins such as BECN1, CCND1, CCND2, CCND3, ELOC and ULK1. Acts as an upstream master regulator of the transition from G1 to S cell phase: AMBRA1 specifically recognizes and binds phosphorylated cyclin-D (CCND1, CCND2 and CCND3, leading to cyclin-D ubiquitination by the DCX(AMBRA1 complex and subsequent degradation. By controlling the transition from G1 to S phase and cyclin-D degradation, AMBRA1 acts as a tumor suppressor that promotes genomic integrity during DNA replication and counteracts developmental abnormalities and tumor growth.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Polyclonal Antibodies
Bioworld Molecular Weight: 130-150KDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of PANC-1, using Ambra1 antibody at 1:890 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 30s., Immunohistochemistry of paraffin-embedded rat lung using AMBRA1 antibody at dilution of 1:100. Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded mouse lung using AMBRA1 antibody at dilution of 1:100. Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol., Immunofluorescence analysis of NIH/3T3 cells using Ambra1 Rabbit pAb at dilution of 1:50. Blue: DAPI for nuclear staining.
Alternative Name: AMBRA1; DCAF3; WDR94; FLJ20294
Immunogen: Recombinant fusion protein of human Ambra1(NP_060219.2).
Conjugate: Unconjugated
Modification: Unmodified