AMPKα2 (Phospho-S345) Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS94020-50, BS94020-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P54646(Human) Q8BRK8(Mouse) Q09137(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF, IHC
All Applications: WB:1:500-1:1,000
ICC:1:50-1:200
IHC:1:50-1:100
Background: Five-prime-AMP-activated protein kinase, known as AMPK, is a heterotrimeric complex that comprises of a catalytic α subunit, and regulatory β and γ. AMPK protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. AMPK is activated by high AMP and low ATP via a mechanism involving allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase (AMPKK), and inhibition of dephosphorylation. Activated AMPK can phosphorylate and regulate in vivo hydroxymethylglutaryl-CoA reductase and acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively. The human AMPKα1 gene maps to chromosome 5p12 and encodes a 548 amino acid protein. The major regulatory site phosphorylated by AMPKK on AMPKα has been identified as Thr 172 within the activation loop between the DFG and APE motifs of the alpha-subunits.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: AMPKα2 (Phospho-S345) polyclonal antibody detects endogenous levels of AMPKα2 protein only when phosphorylated at S345.
Bioworld Molecular Weight: 62 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: ICC staining Phospho-AMPK alpha 2(S345) in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS., ICC staining Phospho-AMPK alpha 2(S345) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: AMPK subunit alpha-2, ACACA kinase, HMGCR kinase, PRKAA2, 5'-AMP-activated protein kinase catalytic subunit alpha-2, AAPK2, ACACA kinase, Acetyl-CoA carboxylase kinase, AMPK alpha 2 chain, AMPK subunit alpha-2, AMPK2, AMPKa2, AMPKalpha2, HMGCR kinase, Hydroxymethylglutaryl-CoA reductase kinase, PRKAA, PRKAA2, Protein kinase AMP activated alpha 2 catalytic subunit, Protein kinase AMP activated catalytic subunit alpha 2,
Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser345 of human AMPK alpha 2.
Conjugate: Unconjugated
Modification: Phosphorylation