Angiopoietin-1 Polyclonal Antibody
Catalogue Numbers: BS67121-50, BS67121-100
Sizes: 50µl, 100µl
Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Swiss-Prot: Q15389
Host: Rabbit
Reactivity: Human, Rat
Applications: WB
All Applications: WB (1/500 - 1/1000)
Background: Binds and activates TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation. Plays an important role in the regulation of angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Required for normal angiogenesis and heart development during embryogenesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. Mediates blood vessel maturation/stability. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Recognizes endogenous levels of Angiopoietin-1 protein.
Extra Notes: Western blot analysis of Angiopoietin-1 expression in K562 (A), Hela (B), Jurkat (C) whole cell lysates.
Bioworld Molecular Weight: ~ 58 kDa
Note: For research use only, not for use in diagnostic procedure.
Alternative Name: KIAA0003; Angiopoietin-1; ANG-1
Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Angiopoietin-1. The exact sequence is proprietary.
Conjugate: Unconjugated
Modification: Unmodified