ANKRD20A3 Polyclonal Antibody
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Catalogue Numbers: BS60731-50, BS60731-100
Sizes: 50µl, 100µl
Swiss-Prot: Q5VUR7
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IHC
Background: Ankyrins are membrane adaptor molecules that play important roles in coupling integral membrane proteins to the spectrin-based cytoskeleton network. Mutations of ankyrin genes lead to severe genetic diseases such as fatal cardiac arrhythmias and hereditary spherocytosis. ANKRD20A (ankyrin repeat domain-containing protein 20A) is an 823 amino acid protein that contains five ANK repeats. The gene encoding ANKRD20A maps to chromosome 9, which consists of about 145 million bases and encodes nearly 900 genes. Considered to play a role in gender determination, deletion of the distal portion of 9p can lead to development of male to female sex reversal, the phenotype of a female with a male X,Y genotype. Hereditary hemorrhagic telangiectasia, which is characterized by harmful vascular defects, and familial dysautonomia are associated with chromosome 9. Also, chromosome 9 is partnered with chromosome 22 in the translocation leading to the aberrant production of BCR-ABL fusion protein often found in leukemias.
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Specificity: ANKRD20A3 polyclonal antibody detects endogenous levels of ANKRD20A3 protein.
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of ANKRD20A3 polyclonal antibody at 1:500 dilution
Lane 1: MCF-7 whole cell lysate
Lane 2: sp2/0 whole cell lysate
Lane 3: PC12 whole cell lysate , Immunohistochemistry (IHC) analyzes of ANKRD20A3 pAb in paraffin-embedded human stomach carcinoma tissue at 1:50,showing membrane and cytoplasmic staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
Bioworld Molecular Weight: ~ 94 kDa
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Alternative Name: Ankyrin repeat domain-containing protein 20A3; ANKRD20A3
Immunogen: A synthetic peptide corresponding to residues in Human ANKRD20A3
Conjugate: Unconjugated
Modification: Unmodified