Annexin A1 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90057-50, BS90057-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P04083 Human;P10107 Mouse;P07150 Rat
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC IHC
All Applications: WB:1:500-1:2,000
ICC:1:50-1:200
IHC:1:200
Background: Plays important roles in the innate immune response as effector of glucocorticoid-mediated responses and regulator of the inflammatory process. Has anti-inflammatory activity. Plays a role in glucocorticoid-mediated down-regulation of the early phase of the inflammatory response. Promotes resolution of inflammation and wound healing. Functions at least in part by activating the formyl peptide receptors and downstream signaling cascades. Promotes chemotaxis of granulocytes and monocytes via activation of the formyl peptide receptors. Contributes to the adaptive immune response by enhancing signaling cascades that are triggered by T-cell activation, regulates differentiation and proliferation of activated T-cells. Promotes the differentiation of T-cells into Th1 cells and negatively regulates differentiation into Th2 cells. Has no effect on unstimulated T cells. Promotes rearrangement of the actin cytoskeleton, cell polarization and cell migration. Negatively regulates hormone exocytosis via activation of the formyl peptide receptors and reorganization of the actin cytoskeleton. Has high affinity for Ca2+ and can bind up to eight Ca2+ ions. Displays Ca2+-dependent binding to phospholipid membranes. Plays a role in the formation of phagocytic cups and phagosomes. Plays a role in phagocytosis by mediating the Ca2+-dependent interaction between phagosomes and the actin cytoskeleton.
Purification and Purity: Protein affinity purified.
Storage and Stability: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Specificity: Annexin A1 polyclonal antibody detects endogenous levels of Annexin A1 protein.
Bioworld Molecular Weight: 39 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Annexin A1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: A431 cell lysate
Lane 2: Rat uterus tissue lysate, ICC staining Annexin A1 in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Annexin A1 polyclonal antibody at a dilution of 1:200 for at least 1 hour at room temperature, washed with PBS. Alexa Fluorc and trade; 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
Alternative Name: Annexin A1, Annexin I, Annexin-1, Calpactin II, Calpactin-2, Chromobindin-9, Lipocortin I, Phospholipase A2 inhibitory protein, p35, ANXA1, ANX1, LPC1, Annexin 1, Annexin A1, Annexin I (lipocortin I), Annexin I, Annexin-1, AnnexinA1, AnnexinI, ANX 1, ANX A1, ANX1, ANXA 1, ANXA1, ANXA1 protein, ANXA1, Calpactin 2, Calpactin II, Calpactin-2, CalpactinII, Chromobindin 9, Chromobindin-9, Chromobindin9, HGNC:533, Lipocortin 1, Lipocortin I, Lipocortin1, LipocortinI, LPC 1, LPC1, p35, Phospholipase A2 inhibitory protein antibody
Immunogen: Recombinant protein within human Annexin A1 aa 150-310.
Conjugate: Unconjugated
Modification: Unmodified