Annexin I (N146) Polyclonal Antibody
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Catalogue Numbers: BS3438-50, BS3438-100
Sizes: 50µl, 100µl
Swiss-Prot: P04083
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: IHC
Background: The annexin family of calcium-binding proteins is composed of at least ten mammalian genes and is characterized by a conserved core domain which binds phospholipids in a Ca2+-dependent manner and a unique amino terminal region which may confer binding specificity. The interaction between these proteins and biological membranes have led to the hypothesis that they are involved in cellular trafficking processes such as endocytosis, exocytosis and cellular adhesion. Annexin I, alternatively referred to as lipocortin, has been implicated as a mediator of the anti-inflammatory response produced by glucocorticoids and as an inhibitor of cPLA2, a potent mediator of inflammation. Annexin II, also called p36, has been shown to exist as a monomer or a heterotetramer, complexed with the S-100-related protein p11. This complex is termed calpactin I. In the tetrameric form, Annexin II is an efficient substrate of the PKC family and Src pp60.
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Specificity: Annexin I (N146) polyclonal antibody detects endogenous levels of Annexin I protein.
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Immunohistochemistry (IHC) analyzes of Annexin I (N146) pAb in paraffin-embedded human breast carcinoma tissue at 1:50,showing membrane and cytoplasmic staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
Bioworld Molecular Weight: ~ 39 kDa
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Alternative Name: Annexin 1; Annexin A1; Annexin I (lipocortin I); Annexin I; Annexin-1; AnnexinA1; AnnexinI; ANX 1; ANX A1; ANX1; ANXA 1; ANXA1; ANXA1 protein; Calpactin 2; Calpactin II; Calpactin-2; CalpactinII; Chromobindin 9; Chromobindin-9; Chromobindin9; Lipocortin 1; Lipocortin I; Lipocortin1; LipocortinI;
LPC 1; LPC1; p35; Phospholipase A2 inhibitory protein;
Immunogen: Synthetic peptide, corresponding to amino acids 111-160 of Human Annexin I.
Conjugate: Unconjugated
Modification: Unmodified