eIF4EBP1 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS80034-50, BS80034-100
Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q13541
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: IHC
All Applications: IHC,1:50 - 1:200
Background: This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Unmodification
Immunogen: Recombinant fusion protein of human eIF4EBP1(NP_004086.1).
Conjugate: Unconjugated
Modification: Unmodified
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Immunohistochemistry of paraffin-embedded human colon carcinoma using eIF4EBP1 antibody at dilution of 1:100.Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.