ELAVL1 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90457-50, BS90457-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q15717(Human) P70372(Mouse)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IHC, IP, FC
All Applications: WB:1:500-1:2,000
IHC:1:100-1:500
FC:1:50-1:100
IP:1:10-1:50
Background: RNA-binding protein that binds to the 3'-UTR region of mRNAs and increases their stability. Involved in embryonic stem cells (ESCs) differentiation: preferentially binds mRNAs that are not methylated by N6-methyladenosine (m6A), stabilizing them, promoting ESCs differentiation. Binds to poly-U elements and AU-rich elements (AREs) in the 3'-UTR of target mRNAs. Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA, and AUUUUUA motifs. Binds preferentially to the 5'-UUUU[AG]UUU-3' motif in vitro. With ZNF385A, binds the 3'-UTR of p53/TP53 mRNA to control their nuclear export induced by CDKN2A. Hence, may regulate p53/TP53 expression and mediate in part the CDKN2A anti-proliferative activity. May also bind with ZNF385A the CCNB1 mRNA.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: ELAVL1 polyclonal antibody detects endogenous levels of ELAVL1 protein.
Immunogen: Recombinant protein
Conjugate: Unconjugated
Modification: Unmodified
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of HuR/ELAVL1 on different lysates using anti-HuR/ELAVL1 antibody at 1/1,000 dilution. POSITIVE CONTROL: Lane 1: Rat spleen tissue Lane 2: Rat heart tisse Lane 3: PC-12 Lane 4: Mouse heart tissue Lane 5: Hela, Immunohistochemical analysis of paraffin-embedded rat lung tissue using anti-HuR/ELAVL1 antibody. Counter stained with hematoxylin.