Galectin 1 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90554-50, BS90554-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P09382(Human) P11762(Rat)
Host: Rabbit
Reactivity: Human, Rat, Mouse
Applications: WB, ICC/IF, IHC, IP, FC
All Applications: WB:1:500-1:1,000
ICC/IF:1:50-1:200
IHC:1:400-1:800
FC:1:50-1:100
IP:1:10-1:50
Background: Galectins are a family of soluble b-galactoside-binding animal lectins that modulate cell-to-cell adhesion and cell-to-extracellular matrix (ECM) interactions and play a role in tumor progression, pre-mRNA splicing and apoptosis. Specifically, Galectin-1 is an autocrine regulator of cell-proliferation that plays a role in the maintenance of G0 and in the control of G2 traverse. Galectin-1, also known as LGALS1, is the protein product of a single gene linked to human chromosome 22q13.1. The Galectin-1 protein contains 135 amino acids, a single internal EcoRI site and a polyadenylation signal. Galectin-1 can localize to both intracellular and extracellular space. Galectin-1 is expressed in human placenta, human lung, HL-6, HepG2 and CEM cells.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: Galectin 1 polyclonal antibody detects endogenous levels of Galectin 1 protein.
Immunogen: Recombinant protein
Conjugate: Unconjugated
Modification: Unmodified
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Galectin 1 on HL-60 cell (1) and human skeletal muscle tissue (2) lysate using anti-Galectin 1 antibody at 1/1,000 dilution., ICC staining Galectin 1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.