HPRT Monoclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: MB66751-50, MB66751-100
Product: Mouse IgM. Supplied in crude ascites with 0.01% sodium azide.
Swiss-Prot: P00492
Host: Mouse
Reactivity: Human
Applications: WB
All Applications: WB (1/500 - 1/1000)
Background: HPRT (hypoxanthine phosphoribosyltransferase 1), also known as HGPRT or HPRT1, is a 218 amino acid cytoplasmic protein that belongs to the purine/pyrimidine phosphoribosyltransferase family. Involved in purine metabolism,HPRT functions as a purine salvage enzyme that catalyzes the conversion of hypoxathine and guanine to their respective mononucleotides (inosine monophosphate and guanosine monophosphate, respectively). HPRT exists as a homotetramer that can bind two magnesium ions as cofactors. Defects in the gene encoding HPRT are the cause of gout and Lesch-Nyhan syndrome (LNS), both of which are characterized by a partial or complete lack of NPRT enzymatic activity. While a partial loss of HPRT enzymatic activity results in a buildup of uric acid (gout), a total loss of enzymatic activity results in hyperuricaemia, mental retardation, choreoathetosis and compulsive selfmutilation, all of which are symptoms associated with LNS. The severity of these diseases suggests an essential role for HPRT in purine metabolism.
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Recognizes endogenous levels of HPRT protein.
Bioworld Molecular Weight: ~ 28 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of HPRT expression in A549 (A) whole cell lysates., ???, ???, ???
HPRT; Hypoxanthine-guanine phosphoribosyltransferase; HGPRT; HGPRTase
Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human HPRT. The exact sequence is proprietary.
Conjugate: Unconjugated
Modification: Unmodified