PHD2/EGLN1 Polyclonal Antibody
Sizes: 50 µl, 100 µl
Catalogue Numbers: BS6184-50, BS6184-100
Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q9GZT9
Host: Rabbit
Reactivity: Human
Applications: IF/ICC
All Applications: IF/ICC,1:50 - 1:200
Background: The protein encoded by this gene catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. HIF is a transcriptional complex that plays a central role in mammalian oxygen homeostasis. This protein functions as a cellular oxygen sensor, and under normal oxygen concentration, modification by prolyl hydroxylation is a key regulatory event that targets HIF subunits for proteasomal destruction via the von Hippel-Lindau ubiquitylation complex. Mutations in this gene are associated with erythrocytosis familial type 3 (ECYT3).
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Polyclonal Antibodies
Immunogen: Recombinant fusion protein of human PHD2/EGLN1(NP_071334.1).
Conjugate: Unconjugated
Modification: Unmodified
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Immunofluorescence analysis of MCF-7 cells using PHD2/EGLN1 antibody. Blue: DAPI for nuclear staining.