Phosphoserine Monoclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: MB65971-50, MB65971-100
Host: Mouse
Reactivity: All
Applications: WB, IHC
All Applications: WB (1/1000 - 1/2000), IHC (1/50 - 1/200)
Background: Changes in the serine/threonine phosphorylation state of a protein in response to various external stimuli can have profound effects on cellular signal transduction, apoptosis and carcinogenesis. The reagents, including phosphorylated protein/peptides, antibodies against the phosphospecific amino acid, are important tools to explore the activation of serine, threonine or tyrosine containing proteins. An aberrant protein phosphorylation is a hallmark of human disease, and the enzymes, particularly protein kinases, which control protein phosphorylation are recognized as a major new drug target family.
Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purification and Purity: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Recognizes endogenous levels of Phosphoserine protein.
Extra Notes: Western blot analysis of Phosphoserine expression in Jurkat (A), mouse brain (B), rat brain (C) whole cell lysates., Immunohistochemical analysis of Phosphotyrosine staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Note: For research use only, not for use in diagnostic procedure.
Immunogen: Purified protein corresponding to Phosphoserine.
Conjugate: Unconjugated
Modification: Unmodified