Phosphotyrosine Monoclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: MB65972-50, MB65972-100
Host: Mouse
Reactivity: All
Applications: WB, IHC
All Applications: WB (1/1000 - 1/2000), IHC (1/50 - 1/200)
Background: Tyrosine phosphorylation plays a key role in cellular signaling. Research studies have shown that in cancer, unregulated tyrosine kinase activity can drive malignancy and tumor formation by generating inappropriate proliferation and survival signals. Antibodies specific for phospho-tyrosine have been invaluable reagents in these studies. The phospho-tyrosine monoclonal antibodies developed by Cell Signaling Technology are exceptionally sensitive tools for studying tyrosine phosphorylation and monitoring tyrosine kinase activity in high throughput drug discovery.
Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purification and Purity: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: ???
Extra Notes: Western blot analysis of Phosphotyrosine expression in Jurkat (A), 293T (B) whole cell lysates., Immunohistochemical analysis of Phosphotyrosine staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Note: For research use only, not for use in diagnostic procedure.
Immunogen: Purified protein corresponding to Phosphotyrosine.
Conjugate: Unconjugated
Modification: Unmodified