SARS-CoV-2 3CLpro Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS80245-50, BS80245-100
Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P0DTC2
Host: Rabbit
Reactivity: SARS-CoV-2
Applications: WB, IF/ICC
All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200
Background: [Replicase polyprotein 1ab]: Multifunctional protein involved in the transcription and replication of viral RNAs. Contains the proteinases responsible for the cleavages of the polyprotein.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Unmodification
Bioworld Molecular Weight: 30-35KDa
Immunogen: Recombinant fusion protein of coronavirus 3CLpro(YP_009725295.1).
Conjugate: Unconjugated
Modification: Unmodified
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of extracts of 293T cells, using SARS-CoV-2 3CLpro antibody at 1:5000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 180s., Immunofluorescence analysis of 293T cells transfected with SARS-CoV-2 3CL pro fusion protein and untreated 293T cells use SARS-CoV-2 3CLpro Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.