SARS-CoV-2 Spike Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS80243-50, BS80243-100
Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P0DTC2
Host: Rabbit
Reactivity: SARS-CoV-2
Applications: DB, WB, IF/ICC, IP
All Applications: DB,1:500 - 1:2000 | WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200 | IP,1:50 - 1:200
Background: The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. The spike is essential for both host specificity and viral infectivity. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It''s been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Unmodification
Bioworld Molecular Weight: 110KDa/180KDa
Immunogen: A synthetic peptide of coronavirus Spike(YP_009724390.1).
Conjugate: Unconjugated
Modification: Unmodified
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of extracts of 293T and transfected 293T-S1, using SARS-CoV-2 Spike antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s., Western blot analysis of extracts of 293T and transfected 293T-S ECD, using SARS-CoV-2 Spike antibody at 1:1000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s., Immobilized Recombinant SARS-COV-2 S1+S2 ECD Protein at 1μg/mL can bind SARS-CoV-2 Spike Rabbit pAb with a linear range of 0.78-50ng/mL., Immunofluorescence analysis of 293T cells transfected with SARS-CoV-2 Spike protein and untreated 293T cells use SARS-CoV-2 Spike Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.