SARS-CoV-2 Spike S2 ECD Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS77919-50, BS77919-100
Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P0DTC2
Host: Rabbit
Reactivity: SARS-CoV-2
Applications: DB, WB, IF/ICC, IP
All Applications: DB,1:500 - 1:2000 | WB,1:500 - 1:2000 | FC,1:50 - 1:200 | IF/ICC,1:50 - 1:200
Background: The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates thisinteraction.The S protein plays key parts in the induction of neutralizing-antibody and T-cellresponses, as well as protective immunity.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Unmodification
Bioworld Molecular Weight: 75KDa
Immunogen: A synthetic peptide of SARS-CoV-2 Spike S2 ECD(YP_009724390.1).
Conjugate: Unconjugated
Modification: Unmodified
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of extracts of normal 293T cells 293T transfected with Spike S2 ECD Protein, using SARS-CoV-2 Spike S2 ECD antibody at 1:5000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 1s., Immobilized Recombinant SARS-COV2 S-ECD-PFS Protein at 1μg/mL can bind SARS-CoV-2 Spike S2 ECD Rabbit pAb with a linear range of 3.12-50ng/mL., Immunofluorescence analysis of 293T cells transfected with SARS-CoV-2 Spike S2 ECD fusion protein and untreated 293T cells use SARS-CoV-2 Spike S2 ECD Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining., Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug SARS-CoV-2 Spike S2 ECD antibody. Western blot was performed from the immunoprecipitate using SARS-CoV-2 Spike S2 ECD antibody at a dilution of 1:5000.