SMYD3 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS91261-50, BS91261-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q9H7B4(Human) Q9CWR2(Mouse)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF, IHC, IP, FC
All Applications: WB:1:500-1:2,000
ICC/IF:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100
Background: Belongs to the histone-lysine methyltransferase family. Contains 1 MYND-type zinc finger. Contains 1 SET domain. Histanes methyltransferase. Methylans 'Lys-4' of histone H3, inducing di- and tri-methylation, but not monomethylation. Plays an important role in transcriptional activation as a member of an RNA polymerase complex. Binds DNA containing 5'-CCCTCC- 3 'or 5'-GAGGGG-3'.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: SMYD3 polyclonal antibody detects endogenous levels of SMYD3 protein.
Immunogen: Recombinant protein
Conjugate: Unconjugated
Modification: Unmodified
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of SMYD3 on different cell/tissue lysate using anti-Profilin 1 antibody at 1/1,000 dilution. Positive control: Lane1: Rat kidney Lane2: PC-12 Lane3: Hela Lane4: Mouse spleen Lane5: NIH-3T3 Lane6: Rat spleen, ICC staining SMYD3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.