Recombinant Human S100-B Protein
Size: 10UG
Catalogue Numbers: RP00699-10
Citations, Manuals and SDS Available upon request.
Description: Recombinant Human S100-B Protein is produced by E. coli expression system. The target protein is expressed with sequence (Met1-Glu92) of human S100 Calcium Binding Protein B/S100B (Accession #P04271) fused with an initial Met at the N-terminus and a 6×His tag at the N-terminus.
Background: S100-B, is an acidic protein with a molecular weight of 21 kDa belonging to the S100 family. S100-B contains two EF-hand-type calcium-binding motifs separated by a hinge region with a hydrophobic cleft. S100-B plays an important role in neurodevelopment, differentiation, and brain construction. S100-B has neuroprotective effects, but at high concentrations S100-B is neurotoxic. Extracellular concentration of S100-B increases following brain damage, which easily penetrates into cerebrospinal fluid in brain damage and then into the blood. S100-B is expressed and produced by astrocytes in vertebrate brains and in the CNS, and the astrocytes are the major cells producing S100-B protein in gray matter, as well as oligodendrocytes are the predominantS100-B in protein producing cells in white matter. The major advantage of using S100-B is that elevations in serum or CSF levels provide a sensitive measure for determining CNS injury at the molecular level before gross changes develop, enabling timely delivery of crucial medical intervention before irreversible damage occurs. In addition, S100-B, which is also present in human melanocytes, is a reliable marker for melanoma malignancy both in bioptic tissue and in serum.
Synonyms: NEF, S100, S100-B, S100beta, S100B, S100 beta
Swiss-Prot: P04271
GeneID: 6285
Species: Human
Purity: > 95% by SDS-PAGE.
Research Areas: Other Recombinant Protein
Expression System: E. coli
Tag: N-His
Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM PBS,150mM NaCl, pH7.4. Contact us for customized product form or formulation.
Endotoxin: < 1 EU/μg of the protein by LAL method.
Symbol: S100-B Storage: Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
Keywords: Recombinant Human S100 Calcium Binding Protein B/S100B Protein, S100B, Protein S100-B, S-100 protein beta chain, S-100 protein subunit beta, S100 calcium-binding protein B, S100b, S100 beta, S100 calcium binding protein B
Research Use Only