Apaf-1 Polyclonal Antibody
Sizes: 50μL, 100μL
Catalogue Numbers: RA21954-50, RA21954-100
Citations, Manuals and MSDS Available upon request.
Background: apoptotic peptidase activating factor 1 (APAF1) Homo sapiens This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC) . Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008],
Condition: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage Instructions: -20°C/1 year
Recommended dilutions: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.
Alternative Names: APAF1; KIAA0413; Apoptotic protease-activating factor 1; APAF-1
Applications: WB; IHC-p; IF (paraffin section) ; ELISA
Species Cross-Reactivity: Human; Mouse; Rat
GeneID (Human): 317
Protein MW (KDa): 135
SWISS: O14727
Source: Rabbit
Research Use Only