Csk Monoclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: RA11022-50, RA11022-100
Citations, Manuals and MSDS Available upon request.
Condition: Ascitic fluid containing 0.03% sodium azide,0.5% BSA, 50%glycerol.
Store at: -20°C/1 year
Recommended Dilution: Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. Flow cytometry: 1/200 - 1/400. ELISA: 1/10000. Not yet tested in other applications.
Purification Technology: Affinity purification
Alternative Names: CSK, Tyrosine-protein kinase CSK, C-Src kinase, Protein-tyrosine kinase CYL
Background: catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,function:Specifically phosphorylates 'Tyr-504' on LCK, which acts as a negative regulatory site. Can also act on the LYN and FYN kinases.,PTM:Autophosphorylation of Tyr-304 occurs only at abnormally high CSK concentrations in vitro.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. CSK subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SH2 domain.,similarity:Contains 1 SH3 domain.,subcellular location:Mainly cytoplasmic, also present in lipid rafts.,subunit:Interacts with PTPN8 (By similarity). Interacts with phosphorylated SIT1, PAG1, LIME1 and TGFB1I1.,tissue specificity:Expressed in lung and macrophages.,
Applications: WB, IF/ICC, FCM, ELISA
Species Cross-Reactivity: Human, Mouse, Rat, Monkey
GeneID (Human): 1445
SWISS: P41240
Source: Mouse
Immunogen: Purified recombinant fragment of human Csk expressed in E. Coli.
Category: Monoclonal
Research Use Only