DNA pol ι Polyclonal Antibody
Sizes: 50μL, 100μL
Catalogue Numbers: RA24258-50, RA24258-100
Citations, Manuals and MSDS Available upon request.
Background: catalytic activity:Deoxynucleoside triphosphate + DNA (n) = diphosphate + DNA (n+1) .,cofactor:Magnesium.,domain:The catalytic core consists of fingers, palm and thumb subdomains, but the fingers and thumb subdomains are much smaller than in high-fidelity polymerases; residues from five sequence motifs of the Y-family cluster around an active site cleft that can accommodate DNA and nucleotide substrates with relaxed geometric constraints, with consequently higher rates of misincorporation and low processivity.,function:Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity.,similarity:Belongs to the DNA polymerase type-Y family.,similarity:Contains 1 umuC domain.,subcellular location:Accumulates at replication forks after DNA damage.,subunit:Binds REV1L (By similarity) . Binds POLH.,tissue specificity:Ubiquitous. Highly expressed in testis.,
Condition: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage Instructions: -20°C/1 year
Recommended dilutions: Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Alternative Names: POLI; RAD30B; DNA polymerase iota; Eta2; RAD30 homolog B
Applications: WB; ELISA
Species Cross-Reactivity: Human; Rat; Mouse
GeneID (Human): 11201
Protein MW (KDa): 85
SWISS: Q9UNA4
Source: Rabbit
Research Use Only