FEN-1 mouse mAb
Sizes: 50µl, 100µl
Catalogue Numbers: RA11199-50, RA11199-100
Citations, Manuals and MSDS Available upon request.
Condition: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Store at: -20°C/1 year
Recommended Dilution: wb dilution 1:1000
Purification Technology: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Alternative Names: DNase IV, FEN-1, FEN1, FEN1_HUMAN, Flap endonuclease 1, Flap structure specific endonuclease 1, Flap structure-specific endonuclease 1, hFEN-1, hFEN1, Maturation factor 1, MF1, Rad2.
Background: flap structure-specific endonuclease 1 (FEN1) Homo sapiens The protein encoded by this gene removes 5&apos, overhanging flaps in DNA repair and processes the 5&apos, ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5&apos, end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions
Applications: WB, IP
Species Cross-Reactivity: Human, Mouse, Rat
GeneID (Human): 2237
SWISS: P39748
Source: Mouse
Immunogen: Purified recombinant human FEN-1 protein fragments expressed in E.coli.
Category: Monoclonal
Research Use Only