FLAG-tag Polyclonal Antibody
Sizes: 50μL, 100μL
Catalogue Numbers: RA20821-50, RA20821-100
Citations, Manuals and MSDS Available upon request.
Background: The DYKDDDDK (FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal FLAG vectors provide an Ek cleavage site for removal of the fusion tag. The FLAG peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the FLAG peptide is more likely to be accessible to antibodies. A FLAG-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on.
Condition: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.05% sodium azide.
Storage Instructions: -20°C/1 year
Recommended dilutions: Western Blot: 1/1000 - 1/3000. ELISA: 1/20000. Not yet tested in other applications.
Alternative Names: DDDDK epitope tag; DDDDK epitope tag; DYKDDDDK epitope tag
Applications: WB; ELISA
Species Cross-Reactivity: Species independent
Source: Rabbit
Research Use Only