HRP-conjugated Mouse Anti-Rabbit IgG Light Chain mAb
Size: 100μg
Catalogue Number: AS126-100
Citations, Manuals and MSDS Available upon request.
Background: Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence) . They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
Category: Secondary Antibodies
Applications: WB, IP, ELISA
Cross-reactivity: Rabbit
Protein Weight: 25 kDa
Observed Molecular Weight: 25 kDa
Immunogen: Recombinant protein (or fragment).
Species: Rabbit
Source: Mouse
Isotype: IgG2b, kappa
Purity: Affinity purification
Storage: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Recommended Dilutions: WB, 1:1000 - 1:3000 IP, 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA, 1:5000-1:10000
NCBI Alias: Rabbit IgG, light chain
Clone Number Info: AMC50031-HRP
Research Use Only