Rabbit Anti Swine IgA (Fc specific) pAb
Size: 100μg
Catalogue Number: AS157-100
Citations, Manuals and MSDS Available upon request.
Background: The reactivity of the antiserum is restricted to the Fc part of the IgA molecule. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against normal swine serum and milk, a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgA. No precipitation reaction is obtained with purified IgG, IgM, and IgG/Fab fragments. In precipitating techniques as immunoelectrophoresis and radial immunodiffusion (Ouchterlony and Mancini) to identify the presence of IgA in swine serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of swine IgA from serum or plasma. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. For such uses labelled and unlabelled cytochemical and immunoassay grade reagents meeting the necessary additional specificity and performance requirements are available.
Category: Secondary Antibodies
Applications: WB, ELISA
Cross-reactivity: Sus scrofa
Protein Weight: 37kDa
Observed Molecular Weight: 55-60kDa
Immunogen: Recombinant protein (or fragment).
Species: Sus scrofa (Pig)
SWISS: K7ZRK0
Alternate Names: IGHA, IgA heavy chian constant region
Source: Rabbit
Isotype: IgG
Purity: Affinity purification
Storage: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH 7.3.
Recommended Dilutions: WB, 1:3000 - 1:18000 ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
NCBI Alias: immunoglobulin alpha heavy chain constant region
Research Use Only