Mouse Anti GST-Tag mAb
Size: 100μg
Catalogue Number: AE001-100
Citations, Manuals and MSDS Available upon request.
Background: Glutathione S-transferases (GSTs), previously known as ligandins, comprise a family of eukaryotic and prokaryotic phase II metabolic isozymes best known for their ability to catalyze the conjugation of the reduced form of glutathione (GSH) to xenobiotic substrates for the purpose of detoxification. The GST family consists of three superfamilies: the cytosolic, mitochondrial, and microsomal—also known as MAPEG—proteins. Members of the GST superfamily are extremely diverse in amino acid sequence, and a large fraction of the sequences deposited in public databases are of unknown function. The Enzyme Function Initiative (EFI) is using GSTs as a model superfamily to identify new GST functions.A GST-tag is often used to separate and purify proteins that contain the GST-fusion protein. The tag is 220 amino acids (roughly 26 KDa) in size, which, compared to tags such as the Myc-tag or the FLAG-tag, is quite large. It can be fused to either the N-terminus or C-terminus of a protein. However, many commercially available sources of GST-tagged plasmids include a thrombin domain for cleavage of the GST tag during protein purification.
Category: Tag Antibodies
Applications: WB, IP, ELISA
Cross-reactivity: Species independent
Protein Weight: 26kDa
Observed Molecular Weight: 27kDa
Immunogen: Recombinant protein (or fragment).
SWISS: P08515
Alternate Names: GST;GST tag;GST-tag
Source: Mouse
Isotype: IgG1
Purity: Affinity purification
Storage: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 1.5% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Recommended Dilutions: WB, 1:2000 - 1:10000 IP, 3μg antibody for 1μg extracts of recombinant protein ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
NCBI Alias: GST
Clone Number Info: AMC0501
Research Use Only