Rabbit Anti His-tag mAb
Size: 100μg
Catalogue Number: AE086-100
Citations, Manuals and MSDS Available upon request.
Background: Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected by using His antibodies.
Category: Tag Antibodies
Applications: WB, IF/ICC, IP, ELISA
Cross-reactivity: Species independent
Observed Molecular Weight: 35 kDa, 58 kDa
Immunogen: Synthetic peptide
Alternate Names: His;His tag;His-tag
Source: Rabbit
Isotype: IgG
Purity: Affinity purification
Storage: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH 7.3.
Recommended Dilutions: WB, 1:10000 - 1:60000 IF/ICC, 1:50 - 1:200 IP, 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
NCBI Alias: His
Clone Number Info: ARC2696
Research Use Only