Viro-MICST Transduction Reagent allows genetically modifying cells directly on magnetic cell purification columns. Based on the i-MICST™ Technology (Integrated Magnetic Immuno-Cell Sorting and Transfection/Transduction), this technology combines cell isolation and genetic modification in one simple and reliable integrated system. For efficient and selective gene delivery to target cells, Viro-MICST can be used in combination with any viral vector and allows you to reduce cell manipulation steps and save time and material.
- Highly efficient
- Rapid, simple and ready to use
- Suitable for all cells
Sizes:
- 250 µL (VMX250)
- 500 µL (VMX500)
- 1 mL (VMX1000)
Storage: +4°C
Shipping Conditions: Room Temperature
Application
- Suitable for all viruses: including AAV, Adenovirus, Lentivirus and Retrovirus.
- Ideal for mammalian cells: Adherent and suspension cells, primary and hard-to-transfect cells, cell lines, sensitive cells.
RECOMMENDED FOR: Transduction/Infection of cells during magnetic cell purification.
For stem cells, please refer to Viro-MICST Stem Transduction Enhancer.
Results
Figure 1: PBMC selective transduction with Viro-MICST. Human PBMC were labeled with either CD45, CD2 or CD15 microbeads. Each condition was then loaded into i) one unmodified MACS® LS column, and selected cells were then infected using standard lentiviral (standard infection) or ii) one unmodified MACS® LS column followed by a MACS® LS column modified with Viro-MICST/ LV.eGFP complexes. Infection efficiency was measured by flow cytometry.
Figure 2: hUC-MSC adenoviral transduction improvedby viroMICST. 1x106 hUC-MSC cells were transduced at different MOI using either standard adenoviral infection procedure or i-MICST. Pictures show hUC-MSCs 2 days after transduction with both procedures at a MOI of 2 (10X).
MACS® is a registered trademark owned by Miltenyi Biotec GmbH and the use of MACS® column is proprietary and patented technology.For any further licensed of MACS® system, please contact Miltenyi.