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Viro-MICST Transduction Reagent
Viro-MICST Transduction Reagent
Viro-MICST Transduction Reagent
Viro-MICST Transduction Reagent

Viro-MICST Transduction Reagent

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$434.33 CAD
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$434.33 CAD
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Viro-MICST Transduction Reagent allows genetically modifying cells directly on magnetic cell purification columns. Based on the i-MICST™ Technology (Integrated Magnetic Immuno-Cell Sorting and Transfection/Transduction), this technology combines cell isolation and genetic modification in one simple and reliable integrated system. For efficient and selective gene delivery to target cells, Viro-MICST can be used in combination with any viral vector and allows you to reduce cell manipulation steps and save time and material.

 

  • Highly efficient
  • Rapid, simple and ready to use
  • Suitable for all cells

 

Sizes:

  • 250 µL (VMX250)
  • 500 µL (VMX500)
  • 1 mL (VMX1000) 

Storage: +4°C
Shipping Conditions: Room Temperature

 

Application

  • Suitable for all viruses: including AAV, Adenovirus, Lentivirus and Retrovirus.
  • Ideal for mammalian cells: Adherent and suspension cells, primary and hard-to-transfect cells, cell lines, sensitive cells.

RECOMMENDED FOR: Transduction/Infection of cells during magnetic cell purification.

For stem cells, please refer to Viro-MICST Stem Transduction Enhancer.

 

Results

Transduction results with Viromicst reagent
Figure 1: PBMC selective transduction with Viro-MICST. Human PBMC were labeled with either CD45, CD2 or CD15 microbeads. Each condition was then loaded into i) one unmodified MACS® LS column, and selected cells were then infected using standard lentiviral (standard infection) or ii) one unmodified MACS® LS column followed by a MACS® LS column modified with Viro-MICST/ LV.eGFP complexes. Infection efficiency was measured by flow cytometry.

 

hUC-MSC adenoviral transduction with ViroMICST Reagent
Figure 2: hUC-MSC adenoviral transduction improvedby viroMICST. 1x106 hUC-MSC cells were transduced at different MOI using either standard adenoviral infection procedure or i-MICST. Pictures show hUC-MSCs 2 days after transduction with both procedures at a MOI of 2 (10X).

 

MACS® is a registered trademark owned by Miltenyi Biotec GmbH and the use of MACS® column is proprietary and patented technology.For any further licensed of MACS® system, please contact Miltenyi.