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3D-Fect™ Transfection Reagent
3D-Fect™ Transfection Reagent
3D-Fect™ Transfection Reagent
3D-Fect™ Transfection Reagent

3D-Fect™ Transfection Reagent

Regular price
$136.00
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$136.00
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3D-Fect™ is suitable for all kinds of scaffolds and cells. This 3D Transfection Reagent is specifically developed to directly transfect cells cultured in 3D scaffolds. This reagent is based on a novel technology that adds a third dimension to cell culture and transfection. 3D matrices not only add a third dimension to cells’ environment, they also allow creating significant differences in cellular characteristics and behaviours.

Because 3D matrices are routinely used in basic research and therapeutic applications, OZ Biosciences has developed 3D-Fect™ Transfection Reagent specific for 3D-scaffolds. In this way, scaffold-based 3D matrices activated with 3D-Fect™/DNA complexes are colonized by cells which are subsequently transfected in situ and in a more natural environment. This method allows studying tissue engineering and regeneration, tumour invasion, neural differentiation, cellular polarization, tissue formation,colonization, neurite growth...


For Gene Silencing applications, please refer to si3D-Fect


  • Highly efficient: cell lines and primary cells
  • Specific for 3D Scaffolds
  • Compatible with all types of nucleic acids
  • Completely biodegradable
  • Long term protein expression
  • Serum Compatible

 

Sizes:

  • 250 µL (TF20250): Good for 65 to 125 transfections with 1 µg of DNA
  • 500 µL (TF20500): Good for 125 to 250 transfections with 1  µg of DNA
  • 1000 µL (TF21000): Good for 250 to 500 transfections with 1 µg of DNA
Storage: +4°C
Shipping Conditions: Room temperature

 

Application

Perfect for all transfections applications in 3D scaffolds such as sponges, matrices, inserts:

    • Tissue engineering
    • Tissue regeneration
    • Tumor invasion
    • Neural differentiation
    • Cellular polarization
    • Tissue formation
    • Colonization
    • Neurite growth

RECOMMENDED FOR: DNA transfection of cells growing in 3D-scaffolds

 

Results

3D Transfection with 3DFect Transfection reagent
 

Figure 1: 3D-Fect allows transfection of a variety of cells (cell lines and primary cells) into 3D-scaffolds: Collagen-based and collagen-derived scaffolds were pre-loaded with complexes formed by 3D-Fect™ (ratio 4:1) and 1 μg of either pVectOZ-GFP (green pictures) or RFP plasmids (red pictures). Cells were then seeded and observed under fluorescence microscopy after 48 h.

 
3D Transfection results with 3DFect Transfection reagent
Figure 2: Alkaline phosphatase secretion of cells transfected into 3D Scaffold: Collagen-derived scaffolds were pre-loaded with complexes formed by 1 μg of pVectOZ-SEAP and 4 μL of 3D-Fect reagent. Cells were then seeded and secreted alkaline phosphatase (SEAP) concentration was measured after 48 h.