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si3D-Fect™ 3D Transfection Reagent
si3D-Fect™ 3D Transfection Reagent
si3D-Fect™ 3D Transfection Reagent

si3D-Fect™ 3D Transfection Reagent

Regular price
$136.00
Regular price
Sale price
$136.00
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si3D-Fect™ is a 3D transfection reagent specifically designed and developed for silencing gene expression in cells cultured on 3D Scaffolds.

3D matrices not only add a third dimension to the cells’ environment, they also allow creating significant differences in cellular phenotype and behaviour. In this way, 3D matrices bearing complexes formed with si3D-Fect™ reagent and siRNA are colonized by cells to be transfected in a more natural environment. si3D-Fect™ reagent associated with 3D matrices allows numerous cell transfections in order to study tissue engineering, tissue regeneration, tumour invasion, neural differentiation, cellular polarization, tissue formation, colonization, neurite growth… 

 

For DNA Transfection, please refer to 3D-Fect

 

  • Highly efficient for gene silencing in 3D matrices
  • Ideal for any 3D scaffolds (sponges, matrices, inserts)
  • Dedicated to short nucleic acid sequences (siRNA, miRNA…)
  • Long term gene silencing
  • Universal (primary cells and cell lines)
  • Serum Compatible
 

Sizes:

  • 250µL (STF40250): Good for 65 to 125 transfections with 50 nM siRNA in 100 µL Gel
  • 500µL (STF40500): Good for 125 to 250 transfections with 50 nM siRNA in 100 µL Gel
  • 1 mL (STF41000): Good for 250 to 500 transfections with 50 nM siRNA in 100 µL Gel


Storage: +4°C
Shipping Conditions:
Room temperature

 

Application 

Perfect for all transfections applications in 3D scaffolds such as sponges, matrices, inserts:

  • tissue engineering
  • tissue regeneration
  • tumour invasion
  • neural differentiation
  • cellular polarization
  • tissue formation
  • colonization
  • neurite growth

RECOMMENDED FOR: Gene silencing of cells growing in 3D-scaffolds


Results

siRNA Transfection in 3D Scaffold results

Figure 1: si3D-Fect™ allows efficient siRNA transfection in 3D Scaffold: Collagen-derived scaffolds were loaded with 50 nM of fluorescently labelled siRNA complexed to 8 µL of si3D-Fect™ transfection reagent. Transfection efficiency on COS-7 and NIH-3T3 cells was observed by fluorescence microscopy after 48 h.

 

gene silencing in 3D-Scaffold results

Figure 2: si3D-Fect™ allows optimal gene silencing in 3D-Scaffold: Collagen-derived scaffolds were loaded with complexes formed by 8 µL si3D-Fect and several concentrations of siRNA (0-50mM) directed against GFP. HeLa-GFP were added and gene silencing was observed 72H after transfection.