Acetyl-Histone H3-K27 Rabbit mAb
Sizes: 20µl, 100µl
Catalogue Numbers: A22264-20, A22264-100
Citations, Manuals and SDS Available upon request.
Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Synonyms: H3t; H3.4; H3/g; H3FT; H3C16; HIST3H3; Acetyl-Histone H3-K27
GeneID: 8290/8350
Swiss-Prot: Q16695/P68431
Reactivity: Human, Mouse, Rat, Other (Wide Range Predicted)
Tested Applications: ELISA, DB, WB, IHC-P, IF/ICC, ChIP, ChIP-seq
Calculated Molecular Weight: 15kDa
Observed Molecular Weight: 17kDa
Immunogen: A synthetic acetylated peptide around K27 of human Histone H3 (NP_003520.1).
Immunogen Species: Human
Host: Rabbit
Isotype: IgG
Purity: Affinity purification
Storage Buffer: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Research Areas: Epigenetics Nuclear Signaling, Protein phosphorylation, Signal Transduction, MAPK-Erk Signaling Pathway
Recommended Dilutions: DB: 1:2000 - 1:20000 | WB: 1:2000 - 1:20000 | IHC-P: 1:50 - 1:200 | IF/ICC: 1:50 - 1:200 | ChIP: 5μg antibody for 5μg-10μg of Chromatin | ChIP-seq: 1:50 - 1:200
Modifications: Acetyl K27
Conjugate: Unconjugated
Cellular Location: Chromosome, Nucleus
Positive Samples: HeLa TSA, NIH/3T3 TSA, C6 TSA
Keywords: 8290, HIST3H3, H3.4, H3/g, H3FT, H3t, histone cluster 3 H3, histone H3.1t, H3 histone family, member T, H3/t, histone 3, H3, Q16695, Histone Cluster 3 H3, H3 Histone Family, Member T, Histone 3, H3/G, H3/T, Histone Cluster 3, Histone H3.1t, Histone H3, HIST1H3A, Acetyl-Histone H3-K27, AcetylHistone H3-K27, Acetyl Histone H3-K27, H3K27ac, H3K27, marker, Chromatin, Chromatin marker
Symbol: H3C1/H3-4
Category: Acetylated Antibodies
Research Use Only