Acetyl-Histone H3-K27 Rabbit mAb
Sizes: 20µl, 100µl
Catalogue Numbers: A2771-20, A2771-100
Citations, Manuals and SDS Available upon request.
Background: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
GeneID: 8290/8350
Swiss Prot: Q16695/P68431
Tested Applications: ELISA, DB, WB, IHC-P, IP, IF/ICC, ChIP
Reactivity: Human, Mouse, Rat, Other (Wide Range Predicted)
Calculated Molecular Weight: 15kDa
Observed Molecular Weight: 17kDa
Immunogen: A synthetic acetylated peptide around K27 of human Histone H3 (P68431).
Immunogen Species: Human
Host: Rabbit
Isotype: IgG
Purity: Affinity purification
Storage Buffer: Store at -20℃. Avoid freeze / thaw cycles.|Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Recommended Dilutions: DB: 1:500 - 1:2000 | WB: 1:500 - 1:2000 | IHC-P: 1:500 - 1:1000 | IP: 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells | IF/ICC: 1:50 - 1:200 | ChIP: 2μg antibody for 5μg-10μg of Chromatin
Research Areas: Epigenetics & Nuclear Signaling, Epigenetic Modifications
Modifications: Acetyl K27
Conjugate: Unconjugated
Positive Samples: HeLa TSA, NIH/3T3 TSA , C6 TSA
Cellular Location: Chromosome, Nucleus
Keywords: CUT&Tag, 8290, HIST3H3, H3.4, H3/g, H3FT, H3t, histone cluster 3 H3, histone H3.1t, H3 histone family, member T, H3/t, histone 3, H3, Q16695, Histone Cluster 3 H3, H3 Histone Family, Member T, Histone 3, H3/G, H3/T, Histone Cluster 3, Histone H3.1t, Histone H3, HIST1H3A, TriMethyl-Histone H3-K27, TriMethylHistone H3-K27, TriMethyl Histone H3-K27, H3K27me3, HIST1H3J, H3/j, H3FJ, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/I, Histone H3/j, Histone H3/k, Histone H3/l, H3K27, anti-H3K27me3, marker, Chromatin, Chromatin marker, Acetyl-Histone H3-K27
Symbol: Histone H3
Category: Acetylated Antibodies
Research Use Only