FEN-1 Rabbit mAb
Sizes: 20µl, 100µl
Catalogue Numbers: A8999-20, A8999-100
Citations, Manuals and SDS Available upon request.
Background: The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.
Synonyms: MF1; RAD2; FEN-1
GeneID: 2237
Swiss-Prot: P39748
Reactivity: Human, Mouse
Calculated Molecular Weight: 43kDa
Observed Molecular Weight: 45kDa
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 250-350 of human FEN-1 (P39748).
Immunogen Species: Human
Host: Rabbit
Isotype: IgG
Purity: Affinity purification
Tested Applications: ELISA, WB, IHC-P
Storage Buffer: Store at -20℃. Avoid freeze / thaw cycles. | Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Recommended Dilutions: WB: 1:500 - 1:2000 | IHC-P: 1:50 - 1:200
Research Areas: Epigenetics Nuclear Signaling, DNA Damage Repair
Modifications: Unmodified
Conjugate: Unconjugated
Positive Samples: 293T, HUVEC, Mouse thymus
Cellular Location: Mitochondrion, Nucleus, Nucleolus, Nucleoplasm
Category: Monoclonal Antibodies
Citations, Manuals and SDS Available upon request.