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Human AchE (Acetylcholinesterase) ELISA Kit

Human AchE (Acetylcholinesterase) ELISA Kit

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Catalogue Number: ELK4614

Alternative Names: ARAChE; N-AChE; YT

Species: Human

Assay Type: Sandwich

Standard: 200 ng/mL

Detection Range: 3.13-200 ng/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids

Assay Length: 3.5h

Research: Signal transduction;Enzyme & Kinase;Metabolic pathway;Hematology;Neuro science;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Acetylcholinesterase(AchE). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Acetylcholinesterase(AchE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Acetylcholinesterase(AchE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Acetylcholinesterase(AchE) in the samples is then determined by comparing the OD of the samples to the standard curve.