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Human ACVR1 (Activin A Receptor Type I) ELISA Kit

Human ACVR1 (Activin A Receptor Type I) ELISA Kit

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$458.00 USD
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Catalogue Number: ELK4461

Alternative Names: ACVR1A; ALK2; ACVRLK2; TSRI; FOP; SKR1; ACTRI; Activin receptor-like kinase 2; Serine/threonine-protein kinase receptor R1; TGF-B superfamily receptor type I

Species: Human

Assay Type: Sandwich

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Length: 3.5h

Research: Signal transduction;Endocrinology;Hormone metabolism;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Activin A Receptor Type I(ACVR1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Activin A Receptor Type I(ACVR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Activin A Receptor Type I(ACVR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Activin A Receptor Type I(ACVR1) in the samples is then determined by comparing the OD of the samples to the standard curve.