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Human ALOX15 (Arachidonate-15-Lipoxygenase) ELISA Kit

Human ALOX15 (Arachidonate-15-Lipoxygenase) ELISA Kit

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Catalogue Number: ELK4753

Alternative Names: LOX15; 15-LOX2; 15-LOX1; 15-LOX; 12-LOX; ALOX15; 15-LO; 15-LPG; LOG15; Lipoxygenase 15; Arachidonate 12-lipoxygenase, leukocyte-type; Arachidonate omega-6 lipoxygenase

Species: Human

Assay Type: Sandwich

Standard: 100 ng/mL

Detection Range: 1.57-100 ng/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Length: 3.5h

Research: Enzyme & Kinase;Metabolic pathway;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Arachidonate-15-Lipoxygenase(ALOX15). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Arachidonate-15-Lipoxygenase(ALOX15). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Arachidonate-15-Lipoxygenase(ALOX15), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Arachidonate-15-Lipoxygenase(ALOX15) in the samples is then determined by comparing the OD of the samples to the standard curve.