Catalogue Number: ELK4499
Alternative Names: Tyrosine-protein kinase BLK; p55-Blk
Assay Type: Sandwich
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids.
Assay Length: 3.5h
Research: Enzyme & Kinase;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to B-Lymphoid Tyrosine Kinase(BLK). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to B-Lymphoid Tyrosine Kinase(BLK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain B-Lymphoid Tyrosine Kinase(BLK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of B-Lymphoid Tyrosine Kinase(BLK) in the samples is then determined by comparing the OD of the samples to the standard curve.