Catalogue Number: ELK4633
Alternative Names: β-Lipotropin; B-LPH
Assay Type: Competitive Inhibition
Standard: 500 pg/mL
Detection Range: 7.82-500 pg/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 2h
Research: Metabolic pathway;Endocrinology;Hormone metabolism;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Beta-Lipotropic Hormone(bLPH) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Beta-Lipotropic Hormone(bLPH). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Beta-Lipotropic Hormone(bLPH) in the samples is then determined by comparing the OD of the samples to the standard curve.