Catalogue Number: ELK4408
Alternative Names: DYRK; DYRK1; HP86; MNB; MNBH; Dual specificity YAK1-related kinase; Protein kinase minibrain homolog
Species: Human
Assay Type: Sandwich
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: tissue homogenates, cell lysates and other biological fluids
Assay Length: 3.5h
Research: Enzyme & Kinase;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A(DYRK1A). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A(DYRK1A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A(DYRK1A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A(DYRK1A) in the samples is then determined by comparing the OD of the samples to the standard curve.