Human HSP90α (Heat Shock Protein HSP 90-alpha) ELISA Kit
Sizes: 48T, 96T
Catalogue Numbers: ELK9054-48, ELK9054-96
Citations, Manuals and MSDS Available upon request.
Alternative Names: FLJ31884; HSP86; HSP89A; HSP90A; HSP90N; HSPC1; HSPCA; HSPCAL1; HSPCAL4; HSPN; Hsp89; Hsp90; LAP2; heat shock 90kD protein 1; alpha|heat shock 90kD protein 1; alpha-like 4|Heat shock protein HSP 90-alpha
Species: Human
Assay Type: Sandwich
Sensitivity: 0.056 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Serum, Plasma, Tissue Homogenates
Assay length: 3.5h
Research Areas: Neuroscience,Stress response
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HSP90α. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HSP90α. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HSP90α, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HSP90α in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only.