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Human RHO (Rhodopsin) ELISA Kit

Human RHO (Rhodopsin) ELISA Kit

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Catalogue Number: ELK4648

Alternative Names: OPN2; RP4; Opsin 2; Rod Pigment; Retinitis Pigmentosa 4; Visual Purple

Species: Human

Assay Type: Sandwich

Standard: 100 ng/mL

Detection Range: 1.57-100 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids

Assay Length: 3.5h

Research: Ophthalmology & Otorhinolaryngology;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rhodopsin(RHO). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rhodopsin(RHO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rhodopsin(RHO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rhodopsin(RHO) in the samples is then determined by comparing the OD of the samples to the standard curve.