[KD Validated] CTCF Rabbit pAb - A18627

[KD Validated] CTCF Rabbit pAb

Sizes: 20µl, 100µl

Catalogue Numbers: A18627-20, A18627-100

Citations, Manuals and SDS Available upon request.

Background: This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Synonyms: MRD21; FAP108; CFAP108; CF

GeneID: 10664

Swiss Prot: P49711

Tested Applications: ELISA, WB, IHC-P, IP, ChIP

Reactivity: Human, Mouse, Rat

Calculated Molecular Weight: 83kDa

Observed Molecular Weight: 140kDa

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human CTCF (NP_006556.1).

Immunogen Species: Human

Host: Rabbit

Isotype: IgG

Purity: Affinity purification

Storage Buffer: Store at -20℃. Avoid freeze / thaw cycles.|Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.

Recommended Dilutions: WB: 1:500 - 1:2000 | IHC-P: 1:50 - 1:200 | IP: 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells | ChIP: 5μg antibody for 10μg-15μg of Chromatin

Research Areas: Epigenetics Nuclear Signaling

Positive Samples: 293T, NIH/3T3, C6

Cellular Location: Chromosome, Nucleus, centromere, Nucleoplasm,

Keywords: sc-5916, sc-15913, sc-15914, sc-28198, 11 zinc finger protein, CCCTC binding factor, CTCF, CTCFL paralog, Transcriptional repressor CTCF, MRD21, CCCTC-binding factor, transcriptional repressor CTCF, 11 zinc finger transcriptional repressor, 11-zinc finger protein, CCCTC-binding factor (zinc finger protein), P49711, 10664, sc5916, sc 5916, sc15913, sc 15913, sc15914, sc 15914, sc28198, sc 28198, CF

Symbol: CTCF

Category: Polyclonal Antibodies

Research Use Only